Expression level of Rubisco activase negatively correlates with Rubisco content in transgenic rice
نویسندگان
چکیده
منابع مشابه
Rubisco, Rubisco activase, and global climate change.
Global warming and the rise in atmospheric CO(2) will increase the operating temperature of leaves in coming decades, often well above the thermal optimum for photosynthesis. Presently, there is controversy over the limiting processes controlling photosynthesis at elevated temperature. Leading models propose that the reduction in photosynthesis at elevated temperature is a function of either de...
متن کاملOverexpression of rubisco activase decreases the photosynthetic CO2 assimilation rate by reducing rubisco content in rice leaves.
The effects of overexpression of Rubisco activase on photosynthesis were studied in transgenic rice expressing barley or maize Rubisco activase. Immunoblot and SDS-PAGE analyses showed that transgenic lines from both gene constructs expressed the foreign Rubisco activase at high levels. The activation state of Rubisco in transgenic lines was slightly higher than that in non-transgenic plants (N...
متن کاملCharacterization of photosystem II photochemistry in transgenic tobacco plants with lowered Rubisco activase content.
Rubisco activase plays an important role in the regulation of CO(2) assimilation. However, it is unknown how activase regulates photosystem II (PSII) photochemistry. To investigate the effects of Rubisco activase on PSII photochemistry, we obtained transgenic tobacco (Nicotiana tabacum) plants with 50% (i7), 25% (i28), and 5% (i46) activase levels as compared to wild type plants by using a gene...
متن کاملCoordinate Expression of Rubisco Activase and Rubisco during Barley Leaf Cell Development.
We have utilized the cellular differentiation gradient and photomorphogenic responses of the first leaf of 7-day-old barley (Hordeum vulgare L.) to examine the accumulation of mRNA and protein encoded by the ribulose-1,5-biphosphate carboxylase holoenzyme (rubisco) activase gene (rca). Previous studies have revealed a pattern of coordinate expression of rubisco subunit polypeptides during devel...
متن کاملPurification and species distribution of rubisco activase.
Ribulose-1,5-bisphosphate carboxylase/oxygenase (rubisco) activase, a soluble chloroplast protein which promotes light-dependent rubisco activation, was partially purified from spinach chloroplasts by ion-exchange and gel-filtration fast protein liquid chromatography. The protein could also be isolated using rate zonal centrifugation in sucrose gradients followed by conventional ion-exchange on...
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ژورنال
عنوان ژورنال: Photosynthesis Research
سال: 2018
ISSN: 0166-8595,1573-5079
DOI: 10.1007/s11120-018-0525-9